Difference between revisions of "Part:BBa K2239014:Design"
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+ | The GDH sequence is cloned from the genome of Bacillus subtilis through PCR amplification, using the primers designed and synthesized based on its sequence. | ||
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===References=== | ===References=== | ||
+ | [1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015. |
Latest revision as of 00:50, 1 November 2017
GDH (glucose dehydrogenas)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
The GDH sequence is cloned from the genome of Bacillus subtilis through PCR amplification, using the primers designed and synthesized based on its sequence.
References
[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.