Difference between revisions of "Part:BBa K2440015"
(→Usage and Biology) |
(→Usage and Biology) |
||
Line 9: | Line 9: | ||
MiRNA locker assembled by using this modularized DNA part was able to bind miR-654 in an Ago2 dependent manner, that is, knockdown of miR-654 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-654 in an Ago2 dependent manner, that is, knockdown of miR-654 was achieved by transfecting cells with miRNA locker. | ||
− | Hsa-miR-654-5p was firstly found in human colorectal cells by miRNA serial analysis of gene expression. And its function is still unknown.1 | + | Hsa-miR-654-5p was firstly found in human colorectal cells by miRNA serial analysis of gene expression. And its function is still unknown.<sup>1</sup> |
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 00:42, 1 November 2017
miR-654 target sequence
It is the target sequence of miR-654, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-654 in an Ago2 dependent manner, that is, knockdown of miR-654 was achieved by transfecting cells with miRNA locker.
Hsa-miR-654-5p was firstly found in human colorectal cells by miRNA serial analysis of gene expression. And its function is still unknown.1
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.