Difference between revisions of "Part:BBa K2239010:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
-
 
  
 +
The CBD tag is added to purify the effective enzymes. When connected with the enzyme DNA and expressed, CBD is able to bind to the cellulose on gauze. After washing off other chemicals in the solution, the enzymes are left on the gauze. As a result, they are successfully immobilized.
  
  
 
===Source===
 
===Source===
  
−
GFP
+
Both the GFP and CBD sequences are retrieved from the GenBank and artificially synthesized.
−
CBD
+
 
  
 
===References===
 
===References===
 +
 +
[1] Etai Shpigel, Arie Goldlust, Gilat Efroni, Amos Avraham, Adi Eshel, Mara Dekel, Oded Shoseyov: Immobilization of Recombinant Heparinase I Fused to Cellulose-Binding Domain, 1999.

Latest revision as of 00:34, 1 November 2017


CBD-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 990
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751
    Illegal SapI site found at 846


Design Notes

The CBD tag is added to purify the effective enzymes. When connected with the enzyme DNA and expressed, CBD is able to bind to the cellulose on gauze. After washing off other chemicals in the solution, the enzymes are left on the gauze. As a result, they are successfully immobilized.


Source

Both the GFP and CBD sequences are retrieved from the GenBank and artificially synthesized.


References

[1] Etai Shpigel, Arie Goldlust, Gilat Efroni, Amos Avraham, Adi Eshel, Mara Dekel, Oded Shoseyov: Immobilization of Recombinant Heparinase I Fused to Cellulose-Binding Domain, 1999.