Difference between revisions of "Part:BBa K2443035"

Latest revision as of 00:10, 1 November 2017

NDK optimized for expression in E. coli

NDK is responsible for the catalysis of the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. It has been Codon optimized for use in Escherichia coli and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 01:01, 19 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 00:10, 1 November 2017 (view source) Harlando (Talk \| contribs)
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−	NDK is responsible for the catalysis of the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal ~~histidine~~ tag. Under the regulation of T7 Promoter, RBS and double terminator.	+	NDK is responsible for the catalysis of the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.