Difference between revisions of "Part:BBa K2443033"

Revision as of 00:08, 1 November 2017

MK optimized for expression in E. coli

myokinase (MK) is a phosphotransferase enzyme that is responsible for the catalyzing the interconversion of adenine nucleotides (ATP, ADP, and AMP). By constantly monitoring phosphate nucleotide levels inside the cell, ADK plays an important role in cellular energy homeostasis. MK has been Codon optimized for use in Escherichia coli and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 726
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 00:55, 19 October 2017 (view source) Registry (Talk \| contribs)		Revision as of 00:08, 1 November 2017 (view source) Harlando (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2443033 short</partinfo>		<partinfo>BBa_K2443033 short</partinfo>

−	myokinase (MK) is a phosphotransferase enzyme that is responsible for the catalyzing the interconversion of adenine nucleotides (ATP, ADP, and AMP). By constantly monitoring phosphate nucleotide levels inside the cell, ADK plays an important role in cellular energy homeostasis. MK has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal ~~histidine~~ tag. Under the regulation of T7 Promoter, RBS and double terminator.	+	myokinase (MK) is a phosphotransferase enzyme that is responsible for the catalyzing the interconversion of adenine nucleotides (ATP, ADP, and AMP). By constantly monitoring phosphate nucleotide levels inside the cell, ADK plays an important role in cellular energy homeostasis. MK has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.