Difference between revisions of "Part:BBa K2443031"

Revision as of 00:07, 1 November 2017

RF3 optimized for expression in E. coli

Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 919
Illegal AgeI site found at 980
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1143

Revision as of 00:50, 19 October 2017 (view source) Registry (Talk \| contribs)		Revision as of 00:07, 1 November 2017 (view source) Harlando (Talk \| contribs) Newer edit →
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−	Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal ~~histidine~~ tag. Under the regulation of T7 Promoter, RBS and double terminator.	+	Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.