Difference between revisions of "Part:BBa K2443029"

 
 
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<partinfo>BBa_K2443029 short</partinfo>
 
<partinfo>BBa_K2443029 short</partinfo>
  
Release factor 1 (RF1) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF1 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.  
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Release factor 1 (RF1) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF1 has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.  
  
  

Latest revision as of 00:05, 1 November 2017


RF1 optimized for expression in E. coli

Release factor 1 (RF1) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF1 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]