Difference between revisions of "Part:BBa K2443024"

 
 
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<partinfo>BBa_K2443024 short</partinfo>
 
<partinfo>BBa_K2443024 short</partinfo>
  
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Initiation factor 2, has been Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal histidine tag and is under the regulation of T7 Promoter, RBS and double terminator.  
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Initiation factor 2, has been Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag and is under the regulation of T7 Promoter, RBS and double terminator.  
  
  

Latest revision as of 00:00, 1 November 2017


IF2 optimized for expression in E. coli

Initiation factor 2, has been Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag and is under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2345
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1700
    Illegal AgeI site found at 311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1031
    Illegal SapI site found at 966