Difference between revisions of "Part:BBa K2295003"

Revision as of 23:45, 31 October 2017

hre (hypoxia response element) ptal (minimalpromoter)

Hypoxia induced factors (HIFs) are transcription factors responding to decreased oxigen levels in the cellular environment. Hypoxia induced factor 1 alpha (HIF1A) is a protein constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. However, in hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (BBa_K2295003) in the nucleus and lead to the expression of the gene of interest (Ziello et al., 2007).

Promoter Characterization

Figure 1: Flow cytometry of hypoxia response element promoter tests.

a) Jurkat, b) HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and c) HRE-pTal:eCFP or d) 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl₂. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 51
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

This part can be seen as an improvement of BBa_K1456004 and BBa_K1720002

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2295003 short</partinfo>
-===similar Parts===
+[[Image:T-FREIBURG-HIF Signaling.png|400px|right|center|
+'''Figure 1: Signaling pathway of HIF1A.'''
+<p>
+</p>]]
+Hypoxia induced factors (HIFs) are transcription factors responding to decreased oxigen levels in the cellular environment.
+Hypoxia induced factor 1 alpha (HIF1A) is a protein constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. However, in hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (<partinfo>BBa_K2295003</partinfo>) in the nucleus and lead to the expression of the gene of interest (Ziello et al., 2007).
 ===Promoter Characterization===