Difference between revisions of "Part:BBa K2109108"

Revision as of 23:36, 31 October 2017

MS2 6XHis (V29I/d1FG)

this part is an improvement upon BBA_K1791003 from the Lethbridge iGEM 2015 submission. It contains a N terminal Hexahistidine tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2109108 short</partinfo>
-this part is an improvement upon BBA_K1791003 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.
+this part is an improvement upon BBA_K1791003 from the Lethbridge iGEM 2015 submission. It contains a N terminal Hexahistidine tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.
 <!-- Add more about the biology of this part here