Difference between revisions of "Part:BBa K2377009"

Revision as of 22:56, 31 October 2017

zrap ribB

Improved target: BBa_K1755301

Improved form:

We replaced a sensor element（Copper-Responsive Two-Component System BBa_K190017）for the Zinc-Responsive Two-Component regulatory system (contains σ54 promoter).

Mechanism of changed sensor The mechanism of Zinc sensor is related to a intrinsic signaling pathway in Ecoli. Which is known that many sensing proteins ZraS immobilized on the membrane of cell. And when Zn2+ show up in periplasmic space, the ions will combine with ZraS and trigger it autophosphorylation. (ZraS*) The phosphorylation signal will transmit from ZraS* to ZraR, then ZraR* come to ZraR(HydG) binding site[1] that will make σ54promoter start downstream transcription. (ZraS* ,ZraR* means activated condition.)

So, we substitute Zinc-Responsive Two-Component regulatory system for the copper one in BBa_K1755301. Successfully construct a new heavy metal sensor.

Function of improved new part Improved new part is BBa_K2377009, which can detect the concentration of Zn2+ and transform it into electric signal.

The downstream protein ribB (3,4-dihydroxy-2-butanone 4-phosphate synthase ,ribB) could catalyze the conversion of D-ribulose 5-phosphate formate to 3,4-dihydroxy-2-butanone 4-phosphate. This step is part of the biosynthesis pathway of riboflavin, an electron carrier in microfunction circuit (MFC).

So when the whole gene circuit works, it will result in a consequence that once the concentration of Zn2+ in environment increased, more riboflavin will be produced to impact the voltage of MFC.

Through this new part we realized monitoring heavy metal Zn2+ automatically, quantitatively and efficiently.

The figure below shows the comparison of the part BBa_K1755301 (left) with the new improved part BBa_K2377009 (right), the riboflavin concentration versus heavy metal ion concentration.

And this is the improve target biobrick designed by 2015 LZU-China iGEM team https://parts.igem.org/Part:BBa_K1755301

Sequence and Features