Difference between revisions of "Part:BBa K2382006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1. | ||
===Source=== | ===Source=== | ||
===References=== | ===References=== | ||
Revision as of 22:51, 31 October 2017
T7 promoter + Thioredoxin-MSMEG_5998 fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 456
Illegal XhoI site found at 462 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 901
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For the purpose of purification through nickel-resin column, we added a 18-bp sequence which can code 6 histidines. In addition, we chose T7 promoter which contains lac operator to express this protein because it can be induced by IPTG. For the terminator, we chose BBa_B0015 because it was commonly used in E. coli. The gene design are shown in Fig. 1.