Difference between revisions of "Part:BBa K2377006"

Latest revision as of 22:46, 31 October 2017

TrxzN, a developed element to generate trxz hpRNA with a intron loop

As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene. So we constructed this composite part to generate trxz hpRNA with a intron loop.

TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts.

Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed.

The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development.What’s more, we noticed that a analysis of promoter-GUS indicated that TRX z is expressed in young tissues with decreasing expression during leaf maturation. So our verification need to be at the young stage of the arabidopsis otherwise the phenotype

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 154
Illegal AgeI site found at 1144
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2377006 short</partinfo>
-As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene. So we constructed this composite part to generate Ecr hpRNA with a intron loop.
+As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene. So we constructed this composite part to generate trxz hpRNA with a intron loop.
+TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts.
+Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed.
+The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development.What’s more, we noticed that a analysis of promoter-GUS indicated that TRX z is expressed in young tissues with decreasing expression during leaf maturation.
+So our verification need to be at the young stage of the arabidopsis otherwise the phenotype
-The ecdysone receptor (ECR) is a such kind of gene, which could up-regulate the vital genes expression through the interaction with ultra-stable protein (USP) under the existence of ecdysteroid hormone.
-Therefore, Ecr gene silencing can effectively increasing the insects mortality and reducing the reproduction rate. Studies have shown when fed aphids with 7.5 ng / ul dsRNA Ecr, the mortality rate up to 70% after 8 days.
-According to the literature, the similarity of Ecr gene sequence between pea aphid and grain aphid was 98.4% and with more than 90% similarity in other kind of aphids, so Ecr is an Ideal RNAi target site to kill aphids with a broad spectrum destruction effect.