Difference between revisions of "Part:BBa K2295003"
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Revision as of 22:14, 31 October 2017
hre (hypoxia response element) ptal (minimalpromoter)
similar Parts
Promoter Characterization
Figure 1: Flow cytometry of hypoxia response element promoter tests.
a) Jurkat, b) HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and c) HRE-pTal:eCFP or d) 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl2. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 51
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
This part can be seen as an improvement of BBa_K1456004 and BBa_K1720002