Difference between revisions of "Part:BBa K2295003"
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===Promoter Characterization===
 
[[Image:T-FREIBURG-HRE Results-50pc.png|900px|thumb|center|
 
[[Image:T-FREIBURG-HRE Results-50pc.png|900px|thumb|center|
 
'''Figure 1: Flow cytometry of hypoxia response element promoter tests. '''
 
'''Figure 1: Flow cytometry of hypoxia response element promoter tests. '''
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2295003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2295003 SequenceAndFeatures</partinfo>
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===References===
 
===References===

Revision as of 22:12, 31 October 2017


hre (hypoxia response element) ptal (minimalpromoter)

similar Parts

Promoter Characterization

Figure 1: Flow cytometry of hypoxia response element promoter tests.

a) Jurkat, b) HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and c) HRE-pTal:eCFP or d) 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl2. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

https://parts.igem.org/Part:BBa_K1456004