Difference between revisions of "Part:BBa K2457001"

(→‎Characterization)
(→‎Characterization)
Line 12: Line 12:
  
 
[[File:BBa K2457001 electropherogram.png]]
 
[[File:BBa K2457001 electropherogram.png]]
 +
 
Figure 1:Sequencing electropherogram from BBa_K2457000.  
 
Figure 1:Sequencing electropherogram from BBa_K2457000.  
  

Revision as of 21:41, 31 October 2017


Standardized Cas9 coding sequence + B0015 terminator

This brick was engineered to be a building block of the standard BioBrick vector for one-step bacterial genome engineering: the ([http://2017.igem.org/Team:Amazonas_Brazil pCRISPeasy]). BBa_K2457001 is composed of the Cas9 coding sequence from Streptococcus pyogenes + BBa_B0015 double terminator. It was rationally designed to be interchangeable AND standardized, providing a BioBrick to attend multiple SynBio purposes. For that matter, the RBS and promoter are easily switchable through 3A Assembly. It’s also standard restriction enzymes safe for all RFCs assemblies.

Usage and Biology

Design

Characterization

BBa K2457001 electropherogram.png

Figure 1:Sequencing electropherogram from BBa_K2457000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]