Difference between revisions of "Part:BBa K2235008"
 
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==Usage and Biology==
 
==Usage and Biology==
  
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The basic part consists of the endo-beta galactosidase enzyme coding sequence with a His-tag C-terminally attached. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucin. The sequence originates fro=m the species Clostridium Perfringens.  
+
The basic part consists of the endo-beta galactosidase enzyme coding sequence with a His-tag C-terminally attached. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucin. The sequence originates from the species Clostridium Perfringens [1].  
  
 
[[File:EBGComp_Mechanism.png|600px|thumb|left|Figure 1: Schematic representation of EBG enzyme reaction mechanism.]]
 
[[File:EBGComp_Mechanism.png|600px|thumb|left|Figure 1: Schematic representation of EBG enzyme reaction mechanism.]]
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==References==
 
==References==
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1. Ashida, H., Maskos, K., Li, S. and Li, Y. (2002). Characterization of a Novel Endo-β-galactosidase Specific for Releasing the Disaccharide GlcNAcα1→4Gal from Glycoconjugates†,‡. Biochemistry, 41(7), pp.2388-2395.
  
 
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Latest revision as of 21:08, 31 October 2017


Endo beta galactosidase

Usage and Biology

The basic part consists of the endo-beta galactosidase enzyme coding sequence with a His-tag C-terminally attached. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucin. The sequence originates from the species Clostridium Perfringens [1].

Figure 1: Schematic representation of EBG enzyme reaction mechanism.




You can find more details about endo-beta galactosidase in its composite part BBa_K2235010.

References

1. Ashida, H., Maskos, K., Li, S. and Li, Y. (2002). Characterization of a Novel Endo-β-galactosidase Specific for Releasing the Disaccharide GlcNAcα1→4Gal from Glycoconjugates†,‡. Biochemistry, 41(7), pp.2388-2395.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]