Revision as of 21:01, 31 October 2017

Constitutive Promoter + RBS + fMt

Figure 1. J23119+RBS+fMt

Introduction

      This sequence is designed for constitutively chelating arsenic ions. We ligated a constitutive promoter(BBa_J23119) with metallothionein (fMT, BBa_K190019) to produce arsenic-binding protein. This metallothionein (fMT) is a kind of chelating protein from Fucus vesiculosus. It can bind both Arsenite (III) and Arsenate(V). This part was first designed by Groningen of iGEM 2009. The part of K190019 consists of fMT and RBS(ribosome--‐binding Site, for initiating translation).

Modifying and Improving the Existed Biobrick

Previous biobrick BBa_K190031 of 2009 iGEM10_Groningen

      The metallothionein (BBa_K190031) is an fMT(BBa_K190019) under control of a low constitutive promotor (BBa_J23109). We failed several times in replicating the ligation of these two parts. After sequencing BBa_K190031, BBa_K190019 and BBa_J23109, we found the constitutive promoter BBa_J23109 has two Spel restriction sites in the prefix.(Figure 2.) Thus, we decided to modify the biobrick by ligating fMT(BBa_K190019) with another constitutive promoter (BBa_J23119).

Figure 2. The sequence of J23109 plasmid.

Sequence and Features