Difference between revisions of "Collections/Cell-Free TX-TL"

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This year our team (Lethbridge Collegiate) has put all the protein components of a cell-free transcription-translation (TX-TL) system in BioBrick standard with affinity purification tags. The idea is to provide an easy to express and purify TX-TL system that anyone can perform and use for protein synthesis testing or teaching.
 
This year our team (Lethbridge Collegiate) has put all the protein components of a cell-free transcription-translation (TX-TL) system in BioBrick standard with affinity purification tags. The idea is to provide an easy to express and purify TX-TL system that anyone can perform and use for protein synthesis testing or teaching.

Revision as of 20:33, 31 October 2017

This collection is a user contributed collection, and is not under curation by iGEM HQ/Registry.

This year our team (Lethbridge Collegiate) has put all the protein components of a cell-free transcription-translation (TX-TL) system in BioBrick standard with affinity purification tags. The idea is to provide an easy to express and purify TX-TL system that anyone can perform and use for protein synthesis testing or teaching.

We sought to make all the TX-TL components available to all iGEM teams so anyone could easily produce this system for cell-free protein expression. In total there are 38 TX-TL proteins that we would like to register as a parts collection.

This collection will grow as we continue to develop a tRNA and ribosome purification strategy in BioBrick standard as well.


More...
NameDescriptionTypeCreated bylengthusesseq
BBa_K2109108MS2 6XHis (V29I/d1FG)CompositeAndy Hudson640  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443000AlaRS optimized for expression in E. coli CompositeLethbridge iGEM 20172862  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443001ArgRS optimized for expression in E. coli CompositeLethbridge iGEM 20171965  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443002AsnRS optimized for expression in E. coli CompositeLethbridge iGEM 20171632  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443003AspRS optimized for expression in E. coli CompositeLethbridge iGEM 20172004  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443004CysRS optimized for expression in E. coli CompositeLethbridge iGEM 20171617  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443005GlnRS optimized for expression in E. coli CompositeLethbridge iGEM 20171899  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443006GluRS optimized for expression in E. coli CompositeLethbridge iGEM 20171650  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443007GlyRS Alpha subunit optimized for expression in E. coli CompositeLethbridge iGEM 20171143  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443008GlyRS Beta subunit optimized for expression in E. coli CompositeLethbridge iGEM 20172301  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443009HisRS optimized for expression in E. coli CompositeLethbridge iGEM 20171506  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443010IleRS optimized for expression in E. coli CompositeLethbridge iGEM 20173048  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443011LeuRS optimized for expression in E. coli CompositeLethbridge iGEM 20172814  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443012LysRS optimized for expression in E. coli CompositeLethbridge iGEM 20171750  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443013MetRS optimized for expression in E. coli CompositeLethbridge iGEM 20172265  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443014PheRS Alpha subunit optimized for expression in E. coli CompositeLethbridge iGEM 20171215  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443015PheRS Beta subunit optimized for expression in E. coli CompositeLethbridge iGEM 20172619  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443016ProRS optimized for expression in E. coli CompositeLethbridge iGEM 20171950  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443017SerRS optimized for expression in E. coli CompositeLethbridge iGEM 20171524  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443018ThrRS optimized for expression in E. coli CompositeLethbridge iGEM 20172160  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443019TrpRS optimized for expression in E. coli CompositeLethbridge iGEM 20171236  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443020TyrRS optimized for expression in E. coli CompositeLethbridge iGEM 20171506  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443021ValRS optimized for expression in E. coli CompositeLethbridge iGEM 20173087  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443022MTF optimized for expression in E. coli CompositeLethbridge iGEM 20171179  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443023IF1 optimized for expression in E. coli CompositeLethbridge iGEM 2017456  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443024IF2 optimized for expression in E. coli CompositeLethbridge iGEM 20172904  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443025IF3 optimized for expression in E. coli CompositeLethbridge iGEM 2017774  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443026EF-G optimized for expression in E. coli CompositeLethbridge iGEM 20172346  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443027EF-Tu optimized for expression in E. coli CompositeLethbridge iGEM 20171419  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443028EF-Ts optimized for expression in E. coli CompositeLethbridge iGEM 20171083  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443029RF1 optimized for expression in E. coli CompositeLethbridge iGEM 20171314  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443030RF2 optimized for expression in E. coli CompositeLethbridge iGEM 20171329  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443031RF3 optimized for expression in E. coli CompositeLethbridge iGEM 20171821  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443032RRF optimized for expression in E. coli CompositeLethbridge iGEM 2017789  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443033MK optimized for expression in E. coli CompositeLethbridge iGEM 2017879  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443034CK optimized for expression in E. coli CompositeLethbridge iGEM 20171377  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443035NDK optimized for expression in E. coli CompositeLethbridge iGEM 2017717  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443036PPiase optimized for expression in E. coli CompositeLethbridge iGEM 2017822  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443037T7 RNA Polymerase optimized for expression in E. coli CompositeLethbridge iGEM 20172886  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443038tRNAPhe-MS2CompositeLethbridge iGEM 2017300  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2443039EYFP-Spinach transcription and translation (TX-TL) measurement deviceMeasurementLethbridge iGEM 20171063  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2722000Exonuclease V, Subunit RecCCodingEnik Baligcs3369  . . . ttaccgctgtttcgctttaatcagtcatga
BBa_K2722001Exonuclease V, Subunit RecDCodingEnik Baligcs1827  . . . ctggcggcgttgtttagttcgcgggaataa
BBa_K27220026 times Chi recombination hotspot to inhibit Exonuclease V (RecBCD) activityDNAEnik Baligcs92  . . . gccactgctggtggccactgctggtggcca
  • To add to this list, please use the category //collections/cell-free_tx-tl