Difference between revisions of "Part:BBa K2460001"
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<partinfo>BBa_K2460001 short</partinfo> | <partinfo>BBa_K2460001 short</partinfo> | ||
| − | This ORF codes for the steptomyces phage φBT1 integrase, which catalyzes a site specific recombination between φBT1 [[Part:BBa_K2460002|attB]] and [[Part:BBa_K2460003|attP]] sequences. | + | This ORF codes for the steptomyces phage φBT1 integrase, which catalyzes a site specific recombination between φBT1 [[Part:BBa_K2460002|attB]] and [[Part:BBa_K2460003|attP]] sequences. This recombinase can be used to flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. |
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| + | =Biological Characterisation= | ||
| + | φBT1 integrase is a member of the large serine recombinases(LSRs) family. LSRs are recombinases encoded by temperate phage genomes, which precisely cut and recombine DNA in a highly controllable and predictable way. The recombinases recognize and bind to attB/P sites as dimers, and cut and recombinate on the attB/P sites,with two dimers forming a Tetramer. The recombinated attB/P sites form attL and attR sites. The recombination is irreversible without the corresponding phage excisionase, making the recombination stable and reliable. | ||
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| + | =Kinetic Characterisation= | ||
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| + | = | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [1]Smith M C. Phage-encoded Serine Integrases and Other Large Serine Recombinases[J]. Microbiology Spectrum, 2015, 3(4). | ||
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Revision as of 20:32, 31 October 2017
φBT1 integrase
This ORF codes for the steptomyces phage φBT1 integrase, which catalyzes a site specific recombination between φBT1 attB and attP sequences. This recombinase can be used to flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
Biological Characterisation
φBT1 integrase is a member of the large serine recombinases(LSRs) family. LSRs are recombinases encoded by temperate phage genomes, which precisely cut and recombine DNA in a highly controllable and predictable way. The recombinases recognize and bind to attB/P sites as dimers, and cut and recombinate on the attB/P sites,with two dimers forming a Tetramer. The recombinated attB/P sites form attL and attR sites. The recombination is irreversible without the corresponding phage excisionase, making the recombination stable and reliable.
Kinetic Characterisation
=
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 180
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 331
Illegal AgeI site found at 1526 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1489