Difference between revisions of "Part:BBa I741018:Experience"
 
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===Applications of BBa_I741018===
 
===Applications of BBa_I741018===
 
Description:
 
Description:
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The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.
 
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XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.
 
  
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Useable in conjunction with promoters:
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Related parts:
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*BBa_I741022 CRP open reading frame
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*BBa_I741023 CRP* open reading frame (modified version of CRP which activates without being bound to cAMP)
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*BBa_I741005 xylR ORF
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If you are having problems cloning with this part consider using one of these promoters:
 
*BBa_I741015 Two way promoter controlled by xylR-xylose and CRP-cAMP
 
*BBa_I741015 Two way promoter controlled by xylR-xylose and CRP-cAMP
 
*BBa_I741017 Two way promoter controlled by xylR-xylose and CRP-cAMP (reverse of BBa_I741015)
 
*BBa_I741017 Two way promoter controlled by xylR-xylose and CRP-cAMP (reverse of BBa_I741015)
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*BBa_I741020 Right facing promoter (for xylF) controlled by xylR, with CRP binding site removed
 
*BBa_I741020 Right facing promoter (for xylF) controlled by xylR, with CRP binding site removed
 
*BBa_I741021 Right facing promoter (for xylA) controlled by xylR, with CRP binding site removed
 
*BBa_I741021 Right facing promoter (for xylA) controlled by xylR, with CRP binding site removed
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Related parts:
 
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*BBa_I741022 CRP open reading frame
 
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*BBa_I741023 CRP* open reading frame (modified version of CRP which activates without being bound to cAMP)
 
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If you are having problems cloning with this part:
 
−
 
  
 
===Promoter halfsites being bound by XylR (modeled by George Khoury PSU iGEM 2007)===
 
===Promoter halfsites being bound by XylR (modeled by George Khoury PSU iGEM 2007)===

Latest revision as of 00:44, 26 October 2007

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I741018

Description: The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.

Related parts:

  • BBa_I741022 CRP open reading frame
  • BBa_I741023 CRP* open reading frame (modified version of CRP which activates without being bound to cAMP)
  • BBa_I741005 xylR ORF

If you are having problems cloning with this part consider using one of these promoters:

  • BBa_I741015 Two way promoter controlled by xylR-xylose and CRP-cAMP
  • BBa_I741017 Two way promoter controlled by xylR-xylose and CRP-cAMP (reverse of BBa_I741015)
  • BBa_I741018 Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
  • BBa_I741019 Right facing promoter (for xylA) controlled by xylR and CRP-cAMP
  • BBa_I741020 Right facing promoter (for xylF) controlled by xylR, with CRP binding site removed
  • BBa_I741021 Right facing promoter (for xylA) controlled by xylR, with CRP binding site removed

Promoter halfsites being bound by XylR (modeled by George Khoury PSU iGEM 2007)

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