Difference between revisions of "Part:BBa K2235011:Design"
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Revision as of 20:25, 31 October 2017
Sialidase enzyme coding composite N terminally attached to secretion system type 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3178
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3117
Illegal XhoI site found at 127 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 649
Illegal NgoMIV site found at 739
Illegal AgeI site found at 2952 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1119
Illegal SapI site found at 2934
Design Notes
Terminator codon was removed using PCR in order to attach the sialidase enzyme composite to the signalling peptide.