Difference between revisions of "Cell-free chassis/Vesicle"
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|<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle <font face=georgia color=#000066 size=4>Chassis<br>description</font>]</center> ||[[Image:Vesicle_Specifications.JPG|center]] | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle <font face=georgia color=#000066 size=4>Chassis<br>description</font>]</center> ||[[Image:Vesicle_Specifications.JPG|center]] | ||
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| − | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle/Preparation_Protocol <font face=georgia color=#000066 size=4>Preparation<br>protocol</font>]</center> || | + | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle/Preparation_Protocol <font face=georgia color=#000066 size=4>Preparation<br>protocol</font>]</center> ||[[Image:Vesicle_Encapsulation.JPG|thumb|400px|center|]]'''Pictorial representation of method for vesicle-encapsulation.''' This method is based on Engineering Asymmetric Vesicles by Sophie Pautot et al (2003) and Toward an artificial cell based on gene expression in vesicles by Vincent Noireaux et al. (2005). |
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|<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle/Preliminary_Testing <font face=georgia color=#000066 size=4>Preliminary<br>Testing</font>]</center> || | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle/Preliminary_Testing <font face=georgia color=#000066 size=4>Preliminary<br>Testing</font>]</center> || | ||
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[[Image:Vesicle_With_Expression.JPG|thumb|600px|center|Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial ''E. coli'' S30 extract together with the DNA constuct [https://parts.igem.org/wiki/index.php/Part:BBa_T9002 BBa_T9002]. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).]] | [[Image:Vesicle_With_Expression.JPG|thumb|600px|center|Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial ''E. coli'' S30 extract together with the DNA constuct [https://parts.igem.org/wiki/index.php/Part:BBa_T9002 BBa_T9002]. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).]] | ||
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==References== | ==References== | ||
Revision as of 00:29, 26 October 2007
Vesicle-Encapsulation
| Cell-Free Systems (back to Intro) |
Introduction |
description |
 |
protocol |
Pictorial representation of method for vesicle-encapsulation. This method is based on Engineering Asymmetric Vesicles by Sophie Pautot et al (2003) and Toward an artificial cell based on gene expression in vesicles by Vincent Noireaux et al. (2005). |
Testing |
Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial E. coli S30 extract together with the DNA constuct BBa_T9002. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).
References
<biblio>
- 1 pmid=12963816
- 2 pmid=16224117
</biblio>