Difference between revisions of "Part:BBa K2235005:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Histag10 from the original literature sequence was replaced by Histag6 in our newly designed sialidase gBlock.  
+
6xHis-tag is N terminally attached to the enzyme sequence.  
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Arthrobacter ureafaciens, add a reference of the article
+
 
  
 
===References===
 
===References===
 +
Egebjerg, J. and Christensen, S. (2005). Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41(3), p.225.

Latest revision as of 19:44, 31 October 2017


Sialidase enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 577
    Illegal NgoMIV site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1047


Design Notes

6xHis-tag is N terminally attached to the enzyme sequence.


Source

References

Egebjerg, J. and Christensen, S. (2005). Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41(3), p.225.