Difference between revisions of "Part:BBa K2361000:Experience"
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The iGEM Groningen 2017 team improved this part by removing the illegal EcoRI site from the original part, which was not codon optimized. This part was also sequenced verified and compatible with RFC10, 23, 25 and 1000. Moreover this part is experimentally validated by successfully silencing GFP using specific guide RNAs and the protein from this part expressed on a pBAD expression vector. As this part is now biobrick compatible, sequenced verified and experimentally validated makes it an improvement over both BBa_K1026001 and BBa_K1994000. | The iGEM Groningen 2017 team improved this part by removing the illegal EcoRI site from the original part, which was not codon optimized. This part was also sequenced verified and compatible with RFC10, 23, 25 and 1000. Moreover this part is experimentally validated by successfully silencing GFP using specific guide RNAs and the protein from this part expressed on a pBAD expression vector. As this part is now biobrick compatible, sequenced verified and experimentally validated makes it an improvement over both BBa_K1026001 and BBa_K1994000. | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 19:00, 31 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2361000
The iGEM Groningen 2017 team improved this part by removing the illegal EcoRI site from the original part, which was not codon optimized. This part was also sequenced verified and compatible with RFC10, 23, 25 and 1000. Moreover this part is experimentally validated by successfully silencing GFP using specific guide RNAs and the protein from this part expressed on a pBAD expression vector. As this part is now biobrick compatible, sequenced verified and experimentally validated makes it an improvement over both BBa_K1026001 and BBa_K1994000.
User Reviews
UNIQe28e3629bf7b8e7f-partinfo-00000000-QINU UNIQe28e3629bf7b8e7f-partinfo-00000001-QINU