Difference between revisions of "Cell-free chassis/Homemade E. coli S30"

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=Homemade ''E. coli'' S30=
 
=Homemade ''E. coli'' S30=
  
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||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems <font face=georgia color=#cc0033 size=4>Cell-Free Systems</font>]
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|[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems <font face=georgia color=#cc0033 size=4>Cell-Free<br>Systems (back to Intro)</font>] || <font face=georgia size=4>Introduction</font>
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||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 <font face=georgia color=#000066 size=4>Chassis description</font>]
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||<font face=georgia color=#000066 size=4>Optimum temperature</font>
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||<font face=georgia color=#000066 size=4>Peak time</font>
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||<font face=georgia color=#000066 size=4>Product stability</font>
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||<font face=georgia color=#000066 size=4>Expression capacity</font>
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||<font face=georgia color=#000066 size=4>Expression lifespan</font>
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|<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 <font face=georgia color=#000066 size=4>Chassis<br>description</font>]</center> || The Homemade ''E. coli'' S30 extract was prepared using '''''E. coli'' strain BL21 (DE3)''' with loss of function mutations in OmpT endoproteinase and lon protease genes. This ''E. coli'' strain can express T7 RNA polymerase under the control of LacI, which is inducible by IPTG <cite>1</cite>.
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The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_E7104 BBa_E7104] has been used to characterize this cell-free chassis
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|<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30/Preparation_Protocol <font face=georgia color=#000066 size=4>Preparation protocol</font>]</center> ||
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|<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30/Preliminary_Testing <font face=georgia color=#000066 size=4>Preliminary Testing</font>]</center> ||
 
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==Description==
 
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[[Image:Homemade_E.coli_S30.png|thumb|185px|left|Flow chart showing summary of protocol for preparing Homemade ''E.coli'' S30 extract based on Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system by Kim DM et al (2006).]]
 
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The Homemade ''E. coli'' S30 extract was prepared using '''''E. coli'' strain BL21 (DE3)''' with loss of function mutations in OmpT endoproteinase and lon protease genes. This ''E. coli'' strain can express T7 RNA polymerase under the control of LacI, which is inducible by IPTG <cite>1</cite>.
 
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Please visit our [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/CFS/Design/Protocols OpenWetWare]for the detailed protocol for preparing Homemade ''E. coli'' S30 extract.
 
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==References==
 
==References==

Revision as of 22:33, 25 October 2007

Homemade E. coli S30

Cell-Free
Systems (back to Intro) 		Introduction
Chassis
description
 The Homemade E. coli S30 extract was prepared using E. coli strain BL21 (DE3) with loss of function mutations in OmpT endoproteinase and lon protease genes. This E. coli strain can express T7 RNA polymerase under the control of LacI, which is inducible by IPTG 1.

The simple constitutive gene expression device BBa_E7104 has been used to characterize this cell-free chassis

Preparation protocol
Preliminary Testing

References

<biblio>

  1. 1 pmid=16797767

</biblio>