Difference between revisions of "Part:BBa K2340005"

Revision as of 17:28, 31 October 2017

Guide RNA construct of human RAB13 (2)

Human RAB13 guide RNA construct with a human U6 promoter and human U6 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 217
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 183
Illegal XhoI site found at 224
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the CRISPR dCas13a from Leptotrichia buccalis (LbuC2c2; BBa_K2340000) to form a CRISPR RNA (crRNA).

The sequence that is complementary to Human RAB13 is AAGTGTTGTTGAAGTTGTCC. This is one of three Human RAB13 guide RNA constructs we have constructed

DNA for this part has not been submitted as a sample to the registry due to time restraints.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2340005 short</partinfo>
---
+Human RAB13 guide RNA construct with a human U6 promoter and human U6 terminator.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><b><font size="+1"> Sequence and Features </font></b></span>
 <partinfo>BBa_K2340005 SequenceAndFeatures</partinfo>
+<b><font size="+1"> Usage and Biology </font></b>
+This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the
+CRISPR dCas13a from <i>Leptotrichia buccalis</i> (LbuC2c2; [https://parts.igem.org/Part:BBa_K2340000 BBa_K2340000]) to form a CRISPR RNA (crRNA).
+The sequence that is complementary to Human RAB13 is AAGTGTTGTTGAAGTTGTCC. This is one of three Human RAB13 guide RNA constructs we have constructed
+<b> DNA for this part has not been submitted as a sample to the registry due to time restraints. </b>