Difference between revisions of "Part:BBa K530008:Experience"
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=== '''Team Kyoto 2017''' ===
 
=== '''Team Kyoto 2017''' ===
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<i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )
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<i>S. cerevisiae</i> expressing GFP from this TDH 3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )
  
 
<b>picture</b>
 
<b>picture</b>
 
Figure1 : Observation of <i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter with a fluorescence microscope.
 
Figure1 : Observation of <i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter with a fluorescence microscope.
  
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<i>B. xylophilus</i> was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the esophagus of <i>B. xylophilus</i> was filled with EGFP. (fig 2 )  
+
<i>B. xylophilus</i> was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of <i>B. xylophilus</i> was filled with EGFP (photo or link). (fig 2 )  
  
 
<b>picture</b>
 
<b>picture</b>
 
Figure2 : <i>B. xylophilus</i> full of EGFP in its esophagus.
 
Figure2 : <i>B. xylophilus</i> full of EGFP in its esophagus.
  
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This means that using TDH3 promoter makes it possible to express a very large amount of EGFP in <i>S. cerevisiae</i>, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding yeast. The above results indicate that ''' [https://parts.igem.org/Part:BBa_K530008  BBa_K530008 (TDH 3 promoter)] ''' and ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' work effectively for new usage that has not been attempted so far. Therefore, we could greatly extend the function of each part.
+
This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in <i>S. cerevisiae</i>, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that ''' [https://parts.igem.org/Part:BBa_K530008  BBa_K530008] ''' and ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003] ''' work effectively as a new usage that has not been attempted so far, greatly extending the function of each part.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 17:24, 31 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K530008

Team Kyoto 2017

S. cerevisiae expressing GFP from this TDH 3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )

picture Figure1 : Observation of S. cerevisiae expressing EGFP by this TDH3 promoter with a fluorescence microscope.

B. xylophilus was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of B. xylophilus was filled with EGFP (photo or link). (fig 2 )

picture Figure2 : B. xylophilus full of EGFP in its esophagus.

This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in S. cerevisiae, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that BBa_K530008 and BBa_K1875003 work effectively as a new usage that has not been attempted so far, greatly extending the function of each part.

User Reviews

UNIQf162b5ee9ec67be7-partinfo-00000000-QINU UNIQf162b5ee9ec67be7-partinfo-00000001-QINU