Difference between revisions of "Part:BBa K2273107"
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The Evaluation Vector (EV) is part of both, the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Evaluation Vector] and the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Signal Peptide Toolbox] of the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br><br> | The Evaluation Vector (EV) is part of both, the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Evaluation Vector] and the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Signal Peptide Toolbox] of the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br><br> | ||
The EV was developed with easily exchangeable units: I) allowing the replacement of the promoter (which drives the system) and II) a multiple cloning site enabling to work with translationally fused composite parts.<br><br> | The EV was developed with easily exchangeable units: I) allowing the replacement of the promoter (which drives the system) and II) a multiple cloning site enabling to work with translationally fused composite parts.<br><br> | ||
| − | + | In summary, the EV was designed to fulfill the following distinct features: | |
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<li>Exchangeable promoter region</li> | <li>Exchangeable promoter region</li> | ||
<li>Insertion of basic or composite parts as expression units</li> | <li>Insertion of basic or composite parts as expression units</li> | ||
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</ol> | </ol> | ||
In the case of the Signal Peptide Toolbox which was designed to increase protein secretion efficiency, a typical composite part consists of a signal peptide (for secretion in bacteria) and a protein of interest. | In the case of the Signal Peptide Toolbox which was designed to increase protein secretion efficiency, a typical composite part consists of a signal peptide (for secretion in bacteria) and a protein of interest. | ||
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===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
Revision as of 16:32, 31 October 2017
Evaluation Vector with PxylA to screen for protein specific secretion efficiency
The Evaluation Vector (EV) is part of both, the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Evaluation Vector] and the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Signal Peptide Toolbox] of the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].
The EV was developed with easily exchangeable units: I) allowing the replacement of the promoter (which drives the system) and II) a multiple cloning site enabling to work with translationally fused composite parts.
In summary, the EV was designed to fulfill the following distinct features:
- Exchangeable promoter region
- Insertion of basic or composite parts as expression units
- Fulfilling the RFC10 and RFC25 BioBrick standard
- Easy cloning and screening procedure in Escherichia coli
In the case of the Signal Peptide Toolbox which was designed to increase protein secretion efficiency, a typical composite part consists of a signal peptide (for secretion in bacteria) and a protein of interest.
Sequence and Features
The DNA sequence of this part has been verified via sequencing before it was sent in.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 247
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1354
Illegal AgeI site found at 1348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 266