Difference between revisions of "Part:BBa K1875003"
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<partinfo>BBa_K1875003 parameters</partinfo>
 
<partinfo>BBa_K1875003 parameters</partinfo>
 
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==<big>''' CONTRIBUTION '''</big>==
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''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] '''  <big>improved</big> this part by connecting this part with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' and added further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
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===Combination with BBa_K530008 (TDH3 promoter)===
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With this combination, this part ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' was used to create the part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] ''' which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats <i>S. cerevisiae</i>. Such usage is innovative and has greatly contributed to our project.

Revision as of 16:30, 31 October 2017


This part contains a GFP reporter

This GFP is used in all of the following plasmids: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


CONTRIBUTION

[http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] improved this part by connecting this part with BBa_K1875003(EGFP) and added further functions. In this improvement, we obtained a new part BBa_K2403003 .

Combination with BBa_K530008 (TDH3 promoter)

With this combination, this part BBa_K1875003(EGFP) was used to create the part BBa_K2403003 which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats S. cerevisiae. Such usage is innovative and has greatly contributed to our project.