Difference between revisions of "Part:BBa K530008:Experience"
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===Applications of BBa_K530008=== | ===Applications of BBa_K530008=== | ||
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<i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 ) | <i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 ) | ||
Revision as of 16:05, 31 October 2017
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Applications of BBa_K530008
Team Kyoto 2017
S. cerevisiae expressing EGFP by this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )
picture Figure1 : Observation of S. cerevisiae expressing EGFP by this TDH3 promoter with a fluorescence microscope.
B. xylophilus was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the esophagus of B. xylophilus was filled with EGFP. (fig 2 )
picture Figure2 : B. xylophilus full of EGFP in its esophagus.
This means that using TDH3 promoter makes it possible to express a very large amount of EGFP in S. cerevisiae, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding yeast. The above results indicate that BBa_K530008 (TDH 3 promoter) and BBa_K1875003(EGFP) work effectively for new usage that has not been attempted so far. Therefore, we could greatly extend the function of each part.
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