Difference between revisions of "Part:BBa K2371012"
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We conducted both PCR check(VF and VR) and enzyme check(XbaI and SpeI)
 
We conducted both PCR check(VF and VR) and enzyme check(XbaI and SpeI)
  
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[[File:Pcr test of eforRed and GFP.png|400px]]
 
<h5>Figure 1.PCR check of eforRed and amliGFP. The expected result of eforRed should be around 1000bp. The insufficient specificity of primers produce another band but the band around 1000bp can present the success of construction. The expected result of amliGFP should be around 1000bo. The control is J23119 biobrick</h5>
 
<h5>Figure 1.PCR check of eforRed and amliGFP. The expected result of eforRed should be around 1000bp. The insufficient specificity of primers produce another band but the band around 1000bp can present the success of construction. The expected result of amliGFP should be around 1000bo. The control is J23119 biobrick</h5>
  
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[[File:Pcr test of eforRed and GFP.png|400px]]
 
  
 +
[[File:RD test of amliRed and GFP.png|400px]]
 
<h5>Figure 2.RD check of eforRed and amlGFP.</h5>
 
<h5>Figure 2.RD check of eforRed and amlGFP.</h5>
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[[File:RD test of amliRed and GFP.png|400px]]
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:56, 31 October 2017


pT7-amilGFP


In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. We add a T7 promotor and a RBS in front of the GFP in order to improve it to be utilized in our T7 report system in cell free system together with N-t7-dCas9(BBa_K2371000) and C-t7-dCas9(BBa_K2371001).

The user need to conduct a PCR to linearize the part before reacting with T7 report system.

We conducted both PCR check(VF and VR) and enzyme check(XbaI and SpeI)

Pcr test of eforRed and GFP.png

Figure 1.PCR check of eforRed and amliGFP. The expected result of eforRed should be around 1000bp. The insufficient specificity of primers produce another band but the band around 1000bp can present the success of construction. The expected result of amliGFP should be around 1000bo. The control is J23119 biobrick


RD test of amliRed and GFP.png

Figure 2.RD check of eforRed and amlGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]