Difference between revisions of "Part:BBa K2365035"
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====PCR and SDS-PAGE==== | ====PCR and SDS-PAGE==== | ||
| − | Showing the size of the target gene. | + | Showing the size of the target gene and the protein. |
[[File:ZHD101 DNA.jpg|400px]] | [[File:ZHD101 DNA.jpg|400px]] | ||
[[File:ZHD101 SDS.jpg|400px]] | [[File:ZHD101 SDS.jpg|400px]] | ||
Revision as of 15:23, 31 October 2017
ZHD101--Zearalenone degrading enzyme (optimized)
the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PCR and SDS-PAGE
Showing the size of the target gene and the protein.
