Difference between revisions of "Part:BBa I742161"

Revision as of 18:56, 25 October 2007

crtI (phytoene dehydrogenase) and crtB (phytoene synthase)

crtIB genes encoding phytoene dehydrogenase and phytoene synthase from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the mutated gene has not been confirmed. This version still contains the two PstI sites. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 500
Illegal PstI site found at 911
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 500
Illegal PstI site found at 911
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 93
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 500
Illegal PstI site found at 911
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 500
Illegal PstI site found at 911
Illegal NgoMIV site found at 2061
Illegal NgoMIV site found at 2191
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I742161 short</partinfo>
-phytoene dehydrogenase and phytoene synthase from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sites.
+''crtIB'' genes encoding phytoene dehydrogenase and phytoene synthase from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the ''crtI'' coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the mutated gene has not been confirmed. This version still contains the two PstI sites.
-Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
+Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the ''Erwinia uredovora'' carotenoid biosynthetic pathway by functional analysis of gene products expressed in ''Escherichia coli''. J. Bacteriol. 172 (12), 6704-6712 (1990)