Difference between revisions of "Part:BBa K2365034"

Revision as of 14:58, 31 October 2017

ZHD101--Zearalenone degrading enzyme

In our developed assay, the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product. In previous studies, ZHD101 has been shown to be successfully expressed by Escherichia coli, Saccharomyces cerevisiae, and rice plants, and the recombinant ZHD101 produced by these genetically modifed organisms efectively degraded ZEN .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 772

PCR Result

This is our Uv mapping result,for this result we set four time point ,Z2 as our time point 1 which are taken after 2 hours’culturation ,shown in pink colour line on the figure and Z4 as time point 2 which are taken after 4 hours’culturation,shown in dark blue line while Z6 as our time point 3 which are taken after 6 hours’culturation shown in pretty blue line. the bottom of the figure is the point 4 which are taken after 8 hours’culturation. We added zearalenone by 1ug/ml and the degrading enzyme start to work.the ZEN is degraded as time goes by.The obvious degrading effect is proved by the height of Uv mapping

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 [[File:ZHD101 uv.png|400px]]
- this is our Uv mapping result,for this result we set four time point ,Z2 as our time point 1 which are taken after 2 hours’culturation ,shown in pink colour line on the figure and Z4 as time  point 2 which are taken after 4 hours’culturation,shown in dark blue line while Z6 as our time point 3 which are taken after 6 hours’culturation shown in pretty blue line. the bottom of the figure is the point 4 which are taken after 8 hours’culturation.
+This is our Uv mapping result,for this result we set four time point ,Z2 as our time point 1 which are taken after 2 hours’culturation ,shown in pink colour line on the figure and Z4 as time  point 2 which are taken after 4 hours’culturation,shown in dark blue line while Z6 as our time point 3 which are taken after 6 hours’culturation shown in pretty blue line. the bottom of the figure is the point 4 which are taken after 8 hours’culturation.
 We added zearalenone by 1ug/ml and the degrading enzyme start to work.the ZEN is degraded as time goes by.The obvious degrading effect is proved by the height of Uv mapping