Difference between revisions of "Part:BBa K2381000"
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<partinfo>BBa_K2381000 short</partinfo> | <partinfo>BBa_K2381000 short</partinfo> | ||
− | pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation ( | + | <p> |
− | This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts <a href="https://parts.igem.org/Part:BBa_K2381001" | + | pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is NAD which is normal in nearly all organisms. |
+ | This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts | ||
+ | <a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381017</a> | ||
<br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker. | <br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker. | ||
+ | </p> | ||
<h3>Reference</h3> | <h3>Reference</h3> |
Revision as of 14:50, 31 October 2017
pMagFast
pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm2). Their light sensing molecule is NAD which is normal in nearly all organisms.
This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts
<a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381017</a>
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
Reference
Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 87
- 1000COMPATIBLE WITH RFC[1000]