Difference between revisions of "Part:BBa K2336027:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None.
 
−
 
  
 +
We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes. We did the point mutation in this part.
  
 
===Source===
 
===Source===
  
−
None.
+
Synthesized by IDT.And we fused 2 sequences(opRF and 3*LBT).
 +
 
  
 
===References===
 
===References===

Revision as of 14:49, 31 October 2017


oprF-GS-FLAG-LBT3-GS-LBT3-GS-LBT3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 915
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes. We did the point mutation in this part.

Source

Synthesized by IDT.And we fused 2 sequences(opRF and 3*LBT).


References