Difference between revisions of "Part:BBa K2273001"

This promoter is under the control of the well-studied regulatory system for competence development in Bacillus subtilis (Figure 1). B. subtilis constantly secretes the ComX pheromone (BBa_K2273003), a 9- to 10-amino acid oligopeptide, as a signalling molecule (a). By rising cell-density, the ComX-concentration in the surrounding medium increases until it reaches a threshold and activates ComP, a membrane-spanning protein kinase (b). This mechanism is called quorum sensing. The kinase reacts to the accumulation of ComX by phosphorylating the response regulator ComA (c) which then works as a transcription factor by binding to several promoters and enhancing their activity (d). (Hamoen et al.)
According to literature the promoter P_rapA is regulated by ComA. Its sequence contains a canonical and non-canonical binding site of ComA (Wolf et al.).
Since the promoter is not only dependent on quorum sensing but also on nutritional conditions, we evaluated the influence of different media (Figure 2). We tested full medium (LB) as well as minimal media (MNGE, MCSE).