Difference between revisions of "Part:BBa K2243003"
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Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.  
 
Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.  
  
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===Usage and Biology===
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Usage:
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D7_Bxb1 gp35 is one of the best expression system. We added RBS D7 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).
 +
 
 +
Biology:
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The Bxb1 coding sequence is the part BBa_K22430012.
 +
 
 +
Design note:
 +
We construct this structure by Gibson Assembly. The D7 RBS is generate by random mutation.
 +
 
 +
Characterize:
 +
We characterize the D7_Bxb1 gp35 in a novel structure, which composed with expressional and transcriptional regulatory elements, and with a corresponding reporter system (BBa_K2243006).
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Revision as of 14:10, 31 October 2017


D7_Bxb1 gp35

Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.


Usage: D7_Bxb1 gp35 is one of the best expression system. We added RBS D7 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).

Biology: The Bxb1 coding sequence is the part BBa_K22430012.

Design note: We construct this structure by Gibson Assembly. The D7 RBS is generate by random mutation.

Characterize: We characterize the D7_Bxb1 gp35 in a novel structure, which composed with expressional and transcriptional regulatory elements, and with a corresponding reporter system (BBa_K2243006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1105
    Illegal NgoMIV site found at 1192
    Illegal AgeI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300