Difference between revisions of "Part:BBa K2365052"

 
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<partinfo>BBa_K2365052 short</partinfo>
 
<partinfo>BBa_K2365052 short</partinfo>
  
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Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.  
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Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.And it is inserted into the expression vector pRS423 and inserted into GFP (S65T), then transformed into Saccharomyces cerevisiae SEY6210 to test the fluorescence intensity of 440-550nm
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:02, 31 October 2017


TPI1 promotor-CYC1 terminator

Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.And it is inserted into the expression vector pRS423 and inserted into GFP (S65T), then transformed into Saccharomyces cerevisiae SEY6210 to test the fluorescence intensity of 440-550nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 318