Difference between revisions of "Part:BBa I742143"

 
 
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<partinfo>BBa_I742143 short</partinfo>
 
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This part consists of the genes sam8 and sam5, encoding tyrosine ammonia lyase and 4-coumarate-3-hydroxylase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzymes acting together are expected to convert tyrosine to caffeic acid. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note that there is a PstI site near the 3' end of the sam5 gene in this version. This will be removed by site directed mutagenesis in due course. This construct was prepared to test activity before undertaking the mutagenesis.  
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This part consists of the genes sam8 and sam5, encoding tyrosine ammonia lyase and 4-coumarate-3-hydroxylase, from the bacterium ''Saccharothrix espanaensis'' DSM 4429. The enzymes acting together are expected to convert tyrosine to caffeic acid. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete ''Saccharothrix espanaensis''' J. Bacteriol. 188 (7), 2666-2673 (2006). Note that there is a PstI site near the 3' end of the sam5 gene in this version. This will be removed by site directed mutagenesis in due course. This construct was prepared to test activity before undertaking the mutagenesis.  
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 18:28, 25 October 2007

sam8+sam5 (tyrosine ammonia lyase + coumarate hydroxylase)

This part consists of the genes sam8 and sam5, encoding tyrosine ammonia lyase and 4-coumarate-3-hydroxylase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzymes acting together are expected to convert tyrosine to caffeic acid. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note that there is a PstI site near the 3' end of the sam5 gene in this version. This will be removed by site directed mutagenesis in due course. This construct was prepared to test activity before undertaking the mutagenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2994
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2994
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 808
    Illegal BamHI site found at 1210
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2994
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2994
    Illegal NgoMIV site found at 551
    Illegal NgoMIV site found at 1595
    Illegal NgoMIV site found at 1603
    Illegal NgoMIV site found at 2353
    Illegal NgoMIV site found at 2526
    Illegal NgoMIV site found at 2928
    Illegal NgoMIV site found at 2932
    Illegal AgeI site found at 498
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 102
    Illegal BsaI site found at 368
    Illegal BsaI.rc site found at 2127