Difference between revisions of "Part:BBa I751400:Design"
(→Design Notes) |
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*[[BBtoTSD adapter]] | *[[BBtoTSD adapter]] | ||
1. Cut Biobrick part with XbaI. | 1. Cut Biobrick part with XbaI. | ||
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2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination) | 2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination) | ||
| + | |||
3. Now you can cut out this part by SalI or BamHI. | 3. Now you can cut out this part by SalI or BamHI. | ||
| − | |||
| − | |||
| + | This makes Biobrick scar-like sequence, "tctagt" in the left and "actaga" in the right. Once oligo is ligated, you can't cut with XbaI anymore. | ||
===Source=== | ===Source=== | ||
Latest revision as of 18:27, 25 October 2007
BioBrick to Tokyo Standard adapter, for XbaI, forward
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 20
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. Cut Biobrick part with XbaI.
2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination)
3. Now you can cut out this part by SalI or BamHI.
This makes Biobrick scar-like sequence, "tctagt" in the left and "actaga" in the right. Once oligo is ligated, you can't cut with XbaI anymore.