Difference between revisions of "Part:BBa K2243015"

Revision as of 13:42, 31 October 2017

Bxb1 attB_pTAC_Bxb1 attP

Reporter of the first latch in bio-flip-flop

Usage

This part is the elementary reporter unit of our Bio-Flip-Flop. It generate another state when the promoter orientation changed along with the recombination of the attB and attP sites.

Biology

J23119 is the consensus sequence of a combinatorial constitutive promoter library family. In nature, Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site. We obtained the promoter, attL and attR sites by oligo synthesis.

Design note

We construct this structure by Gibson Assembly.

Characterize

The characterization have not done yet.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 29
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 49
Illegal BsaI.rc site found at 162

@@ Line 5: / Line 5: @@
 Reporter of the first latch in bio-flip-flop
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===Usage===
+This part is the elementary reporter unit of our Bio-Flip-Flop. It generate another state when the promoter orientation changed along with the recombination of the attB and attP sites.
+===Biology===
+J23119 is the consensus sequence of a combinatorial constitutive promoter library family. In nature, Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site. We obtained the promoter, attL and attR sites by oligo synthesis.
+===Design note===
+We construct this structure by Gibson Assembly.
+===Characterize===
+The characterization have not done yet.
 <!-- -->