Difference between revisions of "Part:BBa K2365034"
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====PCR Result==== | ====PCR Result==== | ||
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Revision as of 13:12, 31 October 2017
ZHD101--Zearalenone degrading enzyme
In our developed assay, the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product. In previous studies, ZHD101 has been shown to be successfully expressed by Escherichia coli, Saccharomyces cerevisiae, and rice plants, and the recombinant ZHD101 produced by these genetically modifed organisms efectively degraded ZEN .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 772
PCR Result
