Difference between revisions of "Part:BBa K2424000"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2424000 short</partinfo> | <partinfo>BBa_K2424000 short</partinfo> | ||
| − | NMT1 promoter used in Schizosaccharomyces Pombe. The promoter is repressible by thiamine. It is the most commonly used promoter in for S. Pombe expression systems. | + | NMT1 promoter used in Schizosaccharomyces Pombe. The promoter is repressible by thiamine. It is the most commonly used promoter in for S. Pombe expression systems. The wildtype sequence of NMT1 contains an XbaI restriction site and is not RFC10-compatible. To get around this, a nucleotide substitution was carried out at |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:04, 31 October 2017
T796A NMT1 promoter
NMT1 promoter used in Schizosaccharomyces Pombe. The promoter is repressible by thiamine. It is the most commonly used promoter in for S. Pombe expression systems. The wildtype sequence of NMT1 contains an XbaI restriction site and is not RFC10-compatible. To get around this, a nucleotide substitution was carried out at
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]