Difference between revisions of "Part:BBa K319039:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| + | This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal. | ||
| + | Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. | ||
| + | In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. | ||
===Applications of BBa_K319039=== | ===Applications of BBa_K319039=== | ||
Revision as of 12:18, 31 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
Applications of BBa_K319039
part1: https://parts.igem.org/Part:BBa_K2267043
part3: https://parts.igem.org/Part:BBa_K2267041
part4: https://parts.igem.org/Part:BBa_K2267042
part6: https://parts.igem.org/Part:BBa_K2267044
part8: https://parts.igem.org/Part:BBa_K2267045
User Reviews
UNIQ5b69f6fdf4f85cb9-partinfo-00000000-QINU UNIQ5b69f6fdf4f85cb9-partinfo-00000001-QINU