Difference between revisions of "Part:BBa K2322002"

 
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<partinfo>BBa_K2322002 short</partinfo>
 
<partinfo>BBa_K2322002 short</partinfo>
  
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https://static.igem.org/mediawiki/parts/5/5f/Part%E7%94%A8no.4.png
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This circuit contains a AOX1 promoter, a ribosome binding site, a ScTPS1 gene, and a terminator. This circuit is used to test the expression of ScTPS1. If the circuit work, the ScTPS1 will express normally in GS115 and affect the amount of trehalose in GS115.
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Name: BBa_K2322002
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AOX1 promoter:
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Sequence of the primer:
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ATGATTTCTGGAATTCGCGGCCGCTTCTA
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GAATGACTACAGATAATGCTAAAGCAC
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Function: AOX1 is a strong promoter in the pichia pastoris. It is highly effective. It can be restricted by glucose, glycerinum, ethyl alcohol. It can be induced by the methanol. In our experiment, our circuit is in an environment containing methanol. In this circuit, it is used to control and increase the expression of ScTPS1, our targeted gene. 
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RBS (Ribosome Binding Site):
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A ribosome binding site is a sequence of mRNA. It is used to make sure the ribosome is on the correct position of the mRNA at the beginning of the translation.
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Sequence: TCACACAGGAAACA
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ScTPS1:
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ATGACTACGGATAACGCTAAGGCGCAACTGACCTCGTCTTCAGGGGGTAACATTATTGTGGTGTCCAACAGGCTTCCCGTGACAATCACTAAAAACAGCAGTACGGGACAGTACGAGTACGCAATGTCGTCCGGAGGGCTGGTCACGGCGTTGGAAGGGTTGAAGAAGACGTACACTTTCAAGTGGTTCGGATGGCCTGGGCTAGAGATTCCTGACGATGAGAAGGATCAGGTGAGGAAGGACTTGCTGGAAAAGTTTAATGCCGTACCCATCTTCCTGAGCGATGAAATCGCAGACTTACACTACAACGGGTTCAGTAATTCTATTCTATGGCCGTTATTCCATTACCATCCTGGTGAGATCAATTTCGACGAGAATGCGTGGTTGGCATACAACGAGGCAAACCAGACGTTCACCAACGAGATTGCTAAGACTATGAACCATAACGATTTAATCTGGGTGCATGATTACCATTTGATGTTGGTTCCGGAAATGTTGAGAGTCAAGATTCACGAGAAGCAACTGCAAAACGTTAAGGTCGGGTGGTTCTGCACACACCATTCCCTTCGAGTGAAATTTACAGAATCTTACCTGTCAGACAAGAGATTTTGAAGGGTGTTTTGAGTTGTGATTTAGTCGGGTTCCACACATACGATTATGCAAGACATTTCTTGTCTTCCGTGCAAAGAGTGCTTAACGTGAACACATTGCCTAATGGGGTGGAATACCAGGGCAGATTCGTTAACGTAGGGGCCTTCCTATCGGTATCGACGTGGACAAGTTCACCGATGGGTTGAAAAAGGAATCCGTACAAAAGAGAATCCAACAATTGAAGGAAACTTTCAAGGGCTGCAAGATCATAGTTGGTGTCGACAGGCTGGATTACATCAAAGGTGTGCCTCAGAAGTTGCACGCCATGGAAGTGTTTCTGAACGAGCATCCAGAATGGAGGGGCAAGGTTGTTCTGGTACAGGTTGCAGTGCCAAGTCGTGGAGATGTGGAAGAGTACCAATATTTAAGATCTGTGGTCAATGAGTTGGTCGGTAGAATCAACGGTCAGTTCGGTACTGTGGAATTCGTCCCCATCCATTTCATGCACAAGTCTATACCATTTGAAGAGCTGATTTCGTTATATGCTGTGAGCGATGTTTGTTTGGTCTCGTCCACCGTGATGGTATGAACTTGGTTCCTACGAATATATTGCTTGCCAAGAAGAAAAGAAAGGTTCCTTAATCCTGAGTGAGTTCACAGGTGCCGCACAATCCTTGAATGGTGCTATTATTGTAAATCCTTGGAACACCGATGATCTTTCTGATGCCATCAACGAGGCCTTGACTTTGCCCGATGTAAAGAAAGAAGTTAACTGGGAAAAACTTTACAAATACTCTCTAAATACACTTCTGCCTTCTGGGGTGAAAATTTCGTCCATGAATTATACAGTACATCATCAAGCTCAACAAGCTCCTCTGCCACCAAAAACTGA
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Terminator:
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Sequence of the primer:
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TGACACCTTGCCCTTTTTTGCCGGACTGCA
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GTTAATTTTTAGTTGCTGAAGATGATGATG
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Function: The terminator is used to control the expression of our targeted gene, ScTPS1.
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Revision as of 12:15, 31 October 2017


-AOX1 promoter-RBS-ScTPS1-

Part%E7%94%A8no.4.png

This circuit contains a AOX1 promoter, a ribosome binding site, a ScTPS1 gene, and a terminator. This circuit is used to test the expression of ScTPS1. If the circuit work, the ScTPS1 will express normally in GS115 and affect the amount of trehalose in GS115.

Name: BBa_K2322002

AOX1 promoter: Sequence of the primer: ATGATTTCTGGAATTCGCGGCCGCTTCTA GAATGACTACAGATAATGCTAAAGCAC Function: AOX1 is a strong promoter in the pichia pastoris. It is highly effective. It can be restricted by glucose, glycerinum, ethyl alcohol. It can be induced by the methanol. In our experiment, our circuit is in an environment containing methanol. In this circuit, it is used to control and increase the expression of ScTPS1, our targeted gene.

RBS (Ribosome Binding Site): A ribosome binding site is a sequence of mRNA. It is used to make sure the ribosome is on the correct position of the mRNA at the beginning of the translation. Sequence: TCACACAGGAAACA

ScTPS1: ATGACTACGGATAACGCTAAGGCGCAACTGACCTCGTCTTCAGGGGGTAACATTATTGTGGTGTCCAACAGGCTTCCCGTGACAATCACTAAAAACAGCAGTACGGGACAGTACGAGTACGCAATGTCGTCCGGAGGGCTGGTCACGGCGTTGGAAGGGTTGAAGAAGACGTACACTTTCAAGTGGTTCGGATGGCCTGGGCTAGAGATTCCTGACGATGAGAAGGATCAGGTGAGGAAGGACTTGCTGGAAAAGTTTAATGCCGTACCCATCTTCCTGAGCGATGAAATCGCAGACTTACACTACAACGGGTTCAGTAATTCTATTCTATGGCCGTTATTCCATTACCATCCTGGTGAGATCAATTTCGACGAGAATGCGTGGTTGGCATACAACGAGGCAAACCAGACGTTCACCAACGAGATTGCTAAGACTATGAACCATAACGATTTAATCTGGGTGCATGATTACCATTTGATGTTGGTTCCGGAAATGTTGAGAGTCAAGATTCACGAGAAGCAACTGCAAAACGTTAAGGTCGGGTGGTTCTGCACACACCATTCCCTTCGAGTGAAATTTACAGAATCTTACCTGTCAGACAAGAGATTTTGAAGGGTGTTTTGAGTTGTGATTTAGTCGGGTTCCACACATACGATTATGCAAGACATTTCTTGTCTTCCGTGCAAAGAGTGCTTAACGTGAACACATTGCCTAATGGGGTGGAATACCAGGGCAGATTCGTTAACGTAGGGGCCTTCCTATCGGTATCGACGTGGACAAGTTCACCGATGGGTTGAAAAAGGAATCCGTACAAAAGAGAATCCAACAATTGAAGGAAACTTTCAAGGGCTGCAAGATCATAGTTGGTGTCGACAGGCTGGATTACATCAAAGGTGTGCCTCAGAAGTTGCACGCCATGGAAGTGTTTCTGAACGAGCATCCAGAATGGAGGGGCAAGGTTGTTCTGGTACAGGTTGCAGTGCCAAGTCGTGGAGATGTGGAAGAGTACCAATATTTAAGATCTGTGGTCAATGAGTTGGTCGGTAGAATCAACGGTCAGTTCGGTACTGTGGAATTCGTCCCCATCCATTTCATGCACAAGTCTATACCATTTGAAGAGCTGATTTCGTTATATGCTGTGAGCGATGTTTGTTTGGTCTCGTCCACCGTGATGGTATGAACTTGGTTCCTACGAATATATTGCTTGCCAAGAAGAAAAGAAAGGTTCCTTAATCCTGAGTGAGTTCACAGGTGCCGCACAATCCTTGAATGGTGCTATTATTGTAAATCCTTGGAACACCGATGATCTTTCTGATGCCATCAACGAGGCCTTGACTTTGCCCGATGTAAAGAAAGAAGTTAACTGGGAAAAACTTTACAAATACTCTCTAAATACACTTCTGCCTTCTGGGGTGAAAATTTCGTCCATGAATTATACAGTACATCATCAAGCTCAACAAGCTCCTCTGCCACCAAAAACTGA

Terminator: Sequence of the primer: TGACACCTTGCCCTTTTTTGCCGGACTGCA GTTAATTTTTAGTTGCTGAAGATGATGATG Function: The terminator is used to control the expression of our targeted gene, ScTPS1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 938
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]