Difference between revisions of "Part:BBa K2377005"

 
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<partinfo>BBa_K2377005 short</partinfo>
 
<partinfo>BBa_K2377005 short</partinfo>
  
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one element to generate hpRNA
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A developed element to generate trxz hpRNA with a normal loop, We found that the hairpin RNA actually possess higher stability and higher efficiency in gene silencing in living organisms including higher plants like Arabidopsis thaliana. In general, the hairpin RNA harbor similar effect with double-stranded RNA, what is different is that there is a loop inserted between the sense arm and anti-sense arm.
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2017.igem.org/wiki/images/d/d4/Lanzhou_Parts_5.png
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:13, 31 October 2017


TrxzL, a developed element to generate trxz hpRNA with loop120 

A developed element to generate trxz hpRNA with a normal loop, We found that the hairpin RNA actually possess higher stability and higher efficiency in gene silencing in living organisms including higher plants like Arabidopsis thaliana. In general, the hairpin RNA harbor similar effect with double-stranded RNA, what is different is that there is a loop inserted between the sense arm and anti-sense arm.

2017.igem.org/wiki/images/d/d4/Lanzhou_Parts_5.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
    Illegal AgeI site found at 1159
  • 1000
    COMPATIBLE WITH RFC[1000]