Difference between revisions of "Part:BBa K2387054"
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In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).  
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In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.02% arabinose (Figure 1).  
  
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[[file:T--Wageningen_UR--mRFP.jpg|400px|center|thumb|<p align="justify">'''Figure 1: Absorbance and fluorescence spectra of mRFP, measured after induction with 0.002% arabinose.'''</p>]]
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[[file:T--Wageningen_UR--mRFP.jpg|400px|center|thumb|<p align="justify">'''Figure 1: Absorbance and fluorescence spectra of mRFP, measured after induction with 0.02% arabinose.'''</p>]]
  
 
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([https://parts.igem.org/Part:BBa_K2387032 BBa_K2387032]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.  
 
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([https://parts.igem.org/Part:BBa_K2387032 BBa_K2387032]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.  

Revision as of 11:19, 31 October 2017


mRFP controlled by inducible araC/pBAD promoter

mRFP is a red monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mRFP presents a maximum pick of excitation at 584 nm and a maximum pick of emission at 607 nm. mRFP was engineered from DsRed to grant it a monomeric conformation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1793
    Illegal AgeI site found at 1905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Functional Parameters

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.02% arabinose (Figure 1).

Figure 1: Absorbance and fluorescence spectra of mRFP, measured after induction with 0.02% arabinose.

This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system (BBa_K2387032). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.

Table 1: Split Proteins.
Protein Part Number (Full length) Part Number (Split Protein)
mRFP BBa_K2387054 BBa_K2387055
eYFP BBa_K2387003 BBa_K2387065
mVenus BBa_K2387045 BBa_K2387046
sfGFP BBa_K2387047 BBa_K2387048
mCerulean BBa_K2387052 BBa_K2387053