Difference between revisions of "Part:BBa K2404020"
 
Line 18: Line 18:
 
<br>
 
<br>
 
1-6: BsmBI<br>
 
1-6: BsmBI<br>
−
16-516: [https://parts.igem.org/Part:BBa_K2404003 PR2]<br>
+
16-513: [https://parts.igem.org/Part:BBa_K2404003 PR2]<br>
−
517-1329: [https://parts.igem.org/Part:BBa_K2404001 TSHH]<br>
+
514-1326: [https://parts.igem.org/Part:BBa_K2404001 TSHH]<br>
−
1334-1339: BsmBI<br>
+
1330-1336: BsmBI<br>
  
  

Latest revision as of 11:12, 31 October 2017


TSH antagonist under control of the PR2 promoter


This construct contains the PR2 promotor, and the TSHH coding sequence. This does not contain a terminator sequence.
This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The PR2 promoter is induced by the plant hormone salicylic acid.


Sequence and Features
1-6: BsmBI
16-513: PR2
514-1326: TSHH
1330-1336: BsmBI



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 242
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1300
    Illegal XhoI site found at 1112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]